Platemaker HTS™

Innovadyne Technologies, Inc.’s Platemaker HTS™ is an instrument designed to offer both high-throughput liquid transfers and reagent additions on one integrated platform. Like the Nanodrop™, Innovadyne's Platemaker HTS incorporates the company's proprietary non-contact dispense technology, coupled with traditional syringe dispensing. The result is a next generation workstation, which enables the dispensing of reagents of widely varying viscosity across a broad volume range (nanoliters to microliters) with excellent precision and accuracy.

The unique combination of a 96-channel, non-contact dispense head coupled to an 8-channel, non-contact/contact dispense capability facilitates a variety of applications from HTS to protein crystallography. Additionally, individual control of each channel allows customized liquid handling on a per well basis enabling applications such as normalization, dispensing of gradients, multiplexing and the dispensing of unique plate maps such as molecular diagnostic assays. The product features the Nanobuilder software system that enables a wide range of applications and data manipulation.

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Features

Non-contact and contact dispensing on one platform
Reliable valve free flow path
Exceptional dynamic range (nanoliters to milliliters)
High-speed sample transfer and reagent addition
Independent operation of each channel (no manifolds)
Fast washing of all tips
Individual channel articulation
High-resolution, 5-plate stage (96, 384, and 1536 formats)
Deep well and crystal plate formats supported
Easy daily maintenance and installation procedures

Applications

PCR

Template transfers
Cocktail addition
Sequencing

HTS

Sample transfer
Reagent addition

Assay Development - Miniaturization

Protein Crystallography

Coarse and fine screen creation
Mother liquor transfer
Protein additions
Hanging and sitting drop

Cell-based Assays

Cell plating
Cell dosing

Bead-based Assays

SPA bead assays
YOx bead assays
Polystyrene bead assays

MALDI Plate Spotting

Oligo QC
Proteins

Performance Specifications

	96-Tip Head	8-Tip Head
Plate Formats	96, 96 deep well, 384, 384 deep well, 1536, 1536 low profile, crystallography plates, custom formats	96, 96 deep well, 384, 384 deep well, 1536, \ 1536 low profile, crystallography plates, custom formats
Plate Positions	5	4
Return to Spot Accuracy	0.1 mm	0.1 mm
Dispense Functions	96-tip transfer via contact or not-contact, reagent addition (non-contact)	Transfer (contact/ non-contact), single tip reagent addition, 8-tip reagent addition
Dispense Modes	All the same volumes, independent	All the same volumes, independent
Aspiration Range	0.1 - 500 µL	0.1 - 500 µL
Dispensing Range	0.1 - 80 µL	0.1 - 40 µL (non-contact) 25 - 500 µL (contact)
Dispensing Precision	CV < 10% at 100 nL CV < 7% at 200 nL CV < 5% at 1 µL	CV < 10% at 100 nL CV < 7% at 200 nL CV < 5% at 1 µL
Dispensing Accuracy	±10% at 100 nL ±7% at 200 nL ±5% at >1 µL	±10% at 100 nL ±7% at 200 nL ±5% at >1 µL
Dead Volume	<1 µL/channel	1.5 µL/channel at 1 µL across 384-well plate
Syringe Capacity	500, 1000 µL	500, 1000 µL
Cycle Time	36 s/transfer, 96 well plate 51 s/transfer, 384 well plate) 111 s/transfer, 1536 well plate (includes aspirate, predispenses, dispense and wash)	20 s/transfer <60 s 1 µL reagent addition (single tip/96-well plate) <17 s 1 µL reagent addition (8 tips/1536-well plate)

Technical Specifications

Dimensions	124.5 cm (49 in) Height, 112 cm (46 in) Width, 63.5 cm (25 in) Depth, 295 kg (650 lb) Weight
Environmental	10 to 40°C, 80% RH
System Gas	Standard grade helium (99.7% pure)
Dispense Tips	304 SS, sapphire
Reagent Trays	Custom, deep well plates
Interface	Ethernet, Nanobuilder software GUI
Automation Control	Nanobuilder component library