Nanodrop™ II

Using the same Nanodrop series fluidics module as the Nanodrop I and ExtY, the Nanodrop II is a complete solution for low volume, high-precision pipetting. The system can be configured with one or two plate positions to enable applications such as assay miniaturization, method development, PCR reaction setup, and protein crystallography. When a single plate nest is used, 4 individual tips can access any well of a standard SBS plate, enabling complex method development and design. The Nanodrop™ II aspirates and dispenses a broad range of liquids including DMSO and features the Nanobuilder© software system that enables a wide range of applications and data manipulation. Innovadyne’s patented technology isolates the solenoid dispense actuators from the sample path. This assures long life and easy, low-cost maintenance.

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Features

Non-contact and contact dispense
Exceptional dynamic range (nanoliter to milliliter)
Aspirate and dispense with individual channel
articulation
Sample transfer and bulk reagent addition on the
same platform
Simple fluid path with no moving parts
FEP, SS, sapphire wetted parts compatible with
commonly used solvents
96-, 384-, and 1536-well plate formats
Deep-well and crystal plates supported
Modular, raised, removable plate nests enable flexible configuration
Easy to program, easy to automate
Simple cleaning and maintenance

Applications

Protein Crystallography

Coarse and fine screen creation
Mother liquor transfer
Protein additions
Hanging and sitting drop

HTS

Sample transfer
Reagent addition

Assay Development - Miniaturization

Cell-based Assays

Cell plating
Cell dosing

Bead-based Assays

SPA bead assays
YOx bead assays
Polystyrene bead assays

PCR

Template transfers
Cocktail addition
Sequencing

MALDI Plate Spotting

Oligo QC
Proteins

Performance Specifications

Plate Formats	96, 96 deep well, 384, 384 deep well, 1536, 1536 low profile, crystallography plates
Plate Positions	2
Return to Spot Accuracy	0.1 mm
Dispense Functions	Compound transfer (contact/non-contact), single tip reagent addition, 8-tip reagent addition
Aspirate Modes	All the same volumes, independent
Dispense Modes	All the same volumes, independent
Aspiration Range	0.1 - 500 µL
Dispensing Range	0.1 - 40 µL (non-contact) 25 - 500 µL (contact)
Dispensing Precision	CV ≤ 10% at 100 nL CV ≤ 7% at 200 nL CV ≤ 5% at 1 µL
Dispensing Accuracy	±10% at 100 nL ±7% at 200 nL ±5% at 1 µL
Dead Volume	1.44 µL/channel at 1 µL across 384-well plate
Syringe Capacity	500, 1000 µL
Cycle Time	20 s/transfer <60 s 1 µL reagent addition (single tip/ 96-well plate) <17 s 1 µL reagent addition (8 tips/ 1536-well plate)

Technical Specifications

Dimension	Fluidics Module	Stage Module
Height	33.0 cm (13 in)	32.3 cm (12.7 in)
Width	27.9 cm (11 in)	24.6 cm (9.7 in)
Depth	44.5 cm (18 in)	53.6 cm (21.1 in)
Weight	11.3 kg (25 lb)	13.4 kg (29.5 lb)

Environmental	10 to 40°C, 80% RH
System Gas	Standard grade helium (99.7% pure)
Dispense Tips	304 SS, sapphire
Reagent Trays	Custom, deep well plates
Interface	Ethernet, Nanobuilder software GUI
Automation Control	Nanobuilder component library