molecular diagnostics solutions at Innovadyne Technologies
Innovadyne's genomics, protein crystallography, and MALDI plate spotting platforms provide reliable and precise low-volume liquid handling for molecular diagnostics protocols. Innovadyne's technology enhances PCR based methodologies by facilitating reaction miniaturization. Reductions in both reagent and sample consumption not only lower costs, but also increase scalability, further enabling genome-wide approaches.
Innovadyne's protein crystallography dispensing platforms deliver high throughput crystallization screens and protein addition, for both sitting drop and hanging drop crystallization. The cost of producing purified protein for high-throughput crystallization screening is expensive, so the use of robots that can dispense the protein without much waste and without denaturation is crucial. The low volume, non-contact dispensing and fast cycle time requirements of high-throughput protein crystallography are an excellent fit for Innovadyne's technology.
For protein characterization with MALDI-TOF mass spectrometry, compounds must be spotted on a flat, metal MALDI target plate. Positional accuracy and precise deposition of the spots is critical. Classical liquid handlers using contact methods are prone to failure due to plate and tip irregularities and poor positional accuracy (typically > 100 µm/axis). Innovadyne's MALDI-TOF dispensing platforms reliably fulfill critical MALDI spotting requirements with precisely manufactured motion control and high-precision non-contact solenoid dispensing.
In the application note "Miniaturizing PCR with the Nanodrop," we compared a traditional liquid handling robot dispensing 10 µL (total volume) amplification reactions in a 384-well format against a Nanodrop assembling 1.25µL reactions using the same components. The data showed that the Nanodrop delivers comparable results while significantly reducing reagent costs and sample consumption.
| Traditional Liquid Handler, 10 µL Assay | Nanodrop, 1.25 µL Assay |
Ct Mean | 28.82 | 29.03 |
Ct St.Dev | 0.22 | 0.3 |
Ct %CV | 0.77 | 1.02 |
Ct Range | 1.2 | 1.86 |
Features (all platforms)
96-tip template and primer transfers and 8-tip system for “cocktail” additions (using Screenmaker or Platemaker)
Able to dispense to “exotic” plate maps
Successful sub-microliter amplification assays
Demonstrated lack of carryover
Allows pre-dilution scale down of reagents for sequencing
Real time QPCR
Sequencing
Easy to clean using bleach or other disinfectants
High throughput plate (kit) replication for molecular diagnostics (Screenmaker or Platemaker)
Options for environmental control (Nanodrop)
PCR applications developed in collaboration with molecular diagnostics customer
Sequencing applications in development with well respected sequencing center
Platforms
Item |
Description |
Plate Positions |
8-Tip Head |
16-Tip Head |
96-Tip Head |
Syringe Channels |
1,4, or 8-Tip Additions to all Wells |
10808 |
1 |
Yes |
- |
- |
8 |
- |
|
10591 |
1 |
Yes |
- |
- |
8 |
Yes |
|
11638 |
2 |
Yes |
- |
- |
8 |
Yes |
|
12056 |
1 or 2 |
- |
Yes |
- |
16 |
Yes |
|
11164 |
5 |
Yes |
- |
Yes |
16 |
Yes |
|
12027 |
5 |
Yes |
- |
Yes |
104 |
Yes |
Software
Item |
Description |
11727 |
|
10591 |
Nanodrop GUI (for Nanodrop only) |
Accessories
Item |
Description |
11193 |
|
|
Eppindorf tube holder (all platforms) |
Specifications
Return-To-Spot Accuracy | 0.1 mm |
Aspiration Range, 8-Tip Head | 0.1-500µL |
Dispensing Range (8-Tip Non-Contact) | 0.1-40µL |
Dispensing Range (96-Tip) | Screenmaker: 0.1-125µL |
Dispensing Precision, 8-Tip Head | CV<10% at 100nL, CV<7% at 200nL, CV<5% at 1µL |
Dispensing Precision, 96-Tip Head | CV<15% at 100nL, CV<10% at 200nL, CV<5% at 1µL |
Dispensing Accuracy, 8-Tip Head | ±10% at 100nL, ±7% at 200nL, ±5% at >1 µL |
Dispensing Accuracy, 96-Tip Head | Screenmaker: ±10% at 100-500nL, ±5% at >1 500nL |
Dead Volume, 8-Tip head | 1.5µL/channel at 1µL across 384-well plate |
Dead Volume, 96-Tip head | <1µL/channel |
Syringe Capacity | 500, 1000µL |
